Troubles with Hi-C?
Our technical article on improving and controlling de novo genome sequencing has been published online in Molecular Ecology. In this article, we provide some clues (never say 'solutions', instead of 'clues', because not that simple!) for improving sample preparation, sequencing, and computation for scaffolding. We also cover some pitfalls of using assessment programs like BUSCO. Real practice of these points has been demonstrated with an example of Madagascar ground gecko (Paroedura picta) to provide the first chromosome-scale genome assembly for the whole family Gekkonidae.
In case you cannot access the link included above, please go to the pre-accepted manuscript deposited at Authorea.